ISOCYPTOX

A novel cell based and ready-to-use concept
for in vitro evaluation of biotransformation-mediated metabolic toxicity

IsoCyp-Tox is an innovative cell-based and ready-to-use kit. The kit consists of 96-well plates seeded with the hepatic cell line HepG2 transiently transduced with individual human CYP450 isoforms, making these cells metabolically competent.

IsoCyp-Tox allows in vitro detection and screening of biotransformation-mediated toxicity of lead and drug targets in just one single experiment.

IsoCyp-Tox is powerful as well as time and cost-saving tool during hit to lead and lead optimization phases in early stage of drug discovery and development.

Imagen ISO ISOCYPTOX

BENEFITS

  • Suitable to current toxicity end-points (MTT,WST,LDH,ATP, etc) without specific know-how.
  • Cost-effective and time saving due to reduction of in-house maintenance and handling of cell cultures.
  • Identification of specific CYPs involved in potential production of toxic metabolites in just one experiment.
  • Designed to allow dose-response studies & IC50 curve fitting at early stage.
  • High reproducibility.
  • Adaptable to automated procedures and High Throughput Screening.
  • Available for just-in-time use.
  • Similar or even higher CYP activity levels than those observed in fresh human hepatocytes.
  • Shipping medium easily to remove by liquefaction at 37ºC.

FEATURES

  • Cell-based.
  • Ready-to-use.
  • Single human CYP450 isoform expression (3A4, 2E1, 1A2, 2A6, 2C9, 2C19).
  • High CYP450 isoform activities.
  • 96-well plate format.
  • Customizable plate design.
  • Exclusive solid shipping medium.

APPLICATIONS 

  • Early detection and screening of potential bioactivation-dependent acute toxicity of target compunds.
  • Identification of CYP isoforms involved in potential production of toxic metabolites.
  • Metabolic activation screening.

 

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