CACOGLOBET
Logo CacoGoblet

A mucus secreting ready-to-use concept for intestinal absorption evaluation

CacoGoblet, a mucus-secreting ready-to-use kit. The kit consists of 24 permeable supports seeded with differentiated polarized Caco-2 and human goblet cells. It, allows in vitro intestinal absorption evaluation of drug targets in a barrier physiologically closer to the intestinal epithelium. CacoGoblet is flexible, since plates can be used up to 5 days after ideal cell barrier differentation at day 21, being a time and cost-saving tool for early stage drug discovery and development.

CacoGoblet cells picture

Benefits

  • Mucus secreting CacoGoblet represents a more predictive model for compounds or formulations with passive diffusion transport pathway.
  • CacoGoblet allows end-users to avoid in-house maintenance and handling of cell cultures, thus reducing operating cost.
  • The kit has been designed to provide a user-friendly and suitable tool for High Throughput automated procedures.
  • CacoGoblet provides a 21-day barrier.
  • Out exclusive shipping medium ensures the stability of barrier properties.
  • High flexibility as the kit useable up to 5 days after 21-day cell barrier differentation.
  • Shipping medium is easy to remove by liquefaction at 37ºC.

Features

  • Cell-based
  • Ready-to-use
  • 24 and 96-HTS insert–integrated plate format
  • Exclusive solid shipping medium

Applications

  • Evaluation of oral absorption efficiency, oral bioavailability and oral toxicity
  • Adaptation to High Throughput Screening of target compounds
  • Study of mechanisms involved in oral and intestinal absorption
  • Suitable for research on new delivery systems

Clinical Importance

  • Goblet cells comprise the second major cell type of the intestinal epithelium.
  • HT29 goblet cells and Caco-2 absorptive cells can be grown as co-cultures with barrier properties and create tight junctions between Caco-2 and HT29.
  • Co-culture model for intestinal drug absorption and transport studies.