In Vitro Cytotoxicity Assays

Identifying potential toxicity at early research stages allows to optimize new compounds development.


Cell-based assays with high flexibility.

time optimisation

Full cell functionality after transportation.

Validation data available and high-quality control standards.


Advancing cell toxicity evaluation with ready-to-use cell-based plates.

CacoReady 96 Transwell


Measure and stratify the cellular toxicity of your new molecular entity by studying TEER resistance and LY Paracellular Flux.

CacoReady 96 Multiwell


Test your compound in terms of cell viability using classical colorimetric techniques.

cover application notes

Say goodbye to outdated colorimetric assays!

Our new application notes on CacoReady plates will show you how to accurately predict and stratify intestinal toxicity using cutting-edge methods like TEER and LY paracellular flux. 


Ensure the safety of your new molecule

Drug-induced gastrointestinal reactions are one of the main causes of post-market drug withdrawal. These adverse effects can potentially be predicted by in vitro assays, since these side effects, such as diarrhea, correlate with intestinal barrier dysfunction. In this regard, the most commonly used models for intestinal drug studies in pharmaceutical research consist of human intestinal epithelial cells, such as the colon adenocarcinoma cell line Caco-2.

Read more

Caco-2 cells have been extensively used over the last thirty years to study drug absorption across the human intestinal mucosa. The cells are seeded at a very specific cell number and differentiated for 21-days on transwell plates containing a permeable membrane support which results in apical and basal compartments, which artificially simulate the intestinal absorption into the bloodstream.

Caco-2 cells are considered an in vitro gold standard  for assessing oral drug absorption. This model provides reliable predictive permeability data either by passive diffusion and/or active transport for hydrophilic to moderately lipophilic compounds and can easily be adapted for high-throughput screening purposes.

The Caco-2 model is also useful to assay active transport of drugs in the central nervous system (CNS) since it expresses two efflux transporters that play a relevant role in the permeation of compounds across the blood brain barrier (BBB).

healthy gut
LEAKY GUT - ReadyCell


Cell toxicity testing and stratification in transwell plates



Frequently Asked Questions

Is the quality control data specific for each kit?

Yes, we provide a concrete quality control for each batch so the customer can have a reference for the kit before and after shipment.

Does ReadyCell shipping medium affect cell lines?

No, ReadyCell’s Shipping Medium consists of a semi-solid culture system specifically designed to preserve cells at room temperature (15-25ºC). This medium maintains a suitable physicochemical environment, keeping adequate moisture conditions for cellular homeostasis and forming a protective cushion that protects cell integrity and functionality during long-distance shipments up to seven days.

Is it possible to add a testing service to the order?

As a general rule, we act as a supplier and do not provide testing services. Nonetheless, feel free to contact us if you wish to test our plates externally. We can direct you to our partners who can assist you in conducting the assay effectively. Additionally, in certain situations, we are open to collaborating to try out new applications of interest to both of us.

What are the differences between transwell, no-transwell and individual transwell?

The integrated Transwell format is recommended for permeability assays by reference, since it enableds an apical-basal polarization. The non-Transwell format is used for other types of tests, including toxicity or cell culture for measurement of protein expression. The individual Transwell is recommended for permeability tests which require the individual mobility of each Transwell for different times or measurements.

What do the current regulations establish on the basis of cell toxicity tests?

According to the European and the American Regulatory Agencies (EMA and FDA), permeability assays employing cultured Caco-2 are the reference model to estimate intestinal drug absorption in humans.

Which are the main reference compounds for CacoReady and what are the differences between them?

The reference compounds used in these tests are categorized as high permeability, low permeability and P-gp substrate. In the case of CacoReady, the main reference high permeability compound is metoprolol (Papp A-B >10 cm/s) and for the low, atenolol (Papp A-B <0.5 cm/s). Digoxin is used as P-gp substrate with a Papp A-B <1 cm/s.

Related news