Transporter Drug Interaction
Efflux Transporters
Optimize the need for and proper design of potential future clinical studies with our range of ready-to-use efflux transporter kits.
DDI screening represents a necessary means not only for assessing and ranking potential drug candidates but also for aiding in the development of mechanisms for predicting and retrospectively explaining in vivo drug performance. Our pre-plated overexpressed transporters allow the study of clinically relevant ligand-receptor interaction effects.
BCRP efflux evaluation for early assessment of drug candidates
24 or 96 transwell insert plates with differentiated MDCK II cells expressing BCRP as well as the parental cell line. BCRP (breast cancer resistance protein; ABCG2) is a high-capacity efflux transporter with broad substrate specificity recognizing large, hydrophobic molecules of either negative or positive charge, organic anions, and sulfate conjugates.
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| REFERENCE | DESCRIPTION |
| KRECE-CTR01 | PreadyPort-CTRL (24 Transwell) |
| KRECE-CTR50 | PreadyPort-CTRL (96 Transwell) |
| KRECE-BCP01 | PreadyPort-BCRP (24 Transwell) |
| KRECE-BCP02 | PreadyPort-BCRP/CTRL 1:1 (24 Transwell) |
| KRECE-BCP50 | PreadyPort-BCRP (96 Transwell) |
| KRECE-BCP51 | PreadyPort-BCRP/CTRL 1:1 (96 Transwell) |
PreadyPort BCRP in the literature
- Miyamoto, R., et al. (2019). The Impact of Endogenous Breast Cancer Resistance Protein on Human P-Glycoprotein-Mediated Transport Assays Using LLC-PK1 Cells Transfected With Human P-Glycoprotein. Journal of Pharmaceutical Sciences, Vol. 108, Issue 3, pp. 1085-1089. https://doi.org/10.1016/j.xphs.2018.10.012
Identify P-gp mediated active efflux
24 or 96 transwell insert plates with differentiated MDCK II cells expressing MDR1 as well as the parental cell line. P-gp not only has a critical role in limiting the entry of drugs into the central nervous system but also plays a part in the intestinal absorption and in the biliary and urinary excretion of drugs.
More info...
| REFERENCE | DESCRIPTION | |
| KRECE-CTR01 | PreadyPort-CTRL (24 Transwell) | |
| KRECE-CTR50 | PreadyPort-CTRL (96 Transwell) | |
| KRECE-MDR01 | PreadyPort-MDR1 (24 Transwell) | |
| KRECE-MDR02 | PreadyPort-MDR1/CTRL 1:1 (24 Transwell) | |
| KRECE-MDR50 | PreadyPort-MDR1 (96 Transwell) | |
| KRECE-MDR51 | PreadyPort-MDR1/CTRL 1:1 (96 Transwell) |
PreadyPort MDR1 in the literature
Screening kit for hepatic metabolism based on transfected MDCK II cells
24 transwell insert plates with differentiated MDCK II cells expressing MRP2 as well as the parental cell line. MRP2 receptor is highly expressed in the liver, showing a relevant role in bilirubin conjugated metabolites. Regulatory agencies are considering receptor relevance in terms of pharmacokinetic activity.
More info...
| REFERENCE | DESCRIPTION |
| KRECE-CTR02 | PreadyPort-CTRL (24 Transwell) |
| KRECE-MRP01 | PreadyPort-MRP2 (24 Transwell) |
| KRECE-MRP02 | PreadyPort-MRP2/CTRL 1:1 (24 Transwell) |
ReadyCell ready-to-use cell monolayer kits benefits
- No in-house cell culture maintenance nor cell licensing costs
- Shipments worldwide to any location at room temperature thanks to our patented technology
- High flexibility
- High reproducibility within and between manufacturing batches
- Cell-based assays ready-to-use
- Full cell functionality after transportation
- Highly predictive and useful in the in vitro to in vivo progression
- Validation data available and high quality-control standards
- Technical support during the assay and in bio-analytics
Related News
Renal elimination of drugs: The role of kidney transporters
Understanding the role of these transporters in the clearance of new molecular entities (NME) is normally performed in-vitro at the early stages of drug development.
Assessment of Drug Permeation across the Blood-Brain-Barrier (BBB) in preclinical research
The endothelial cells that conform the BBB limit the passage of blood circulating endogenous substrates and larger molecules to the brain. This intrinsic protective role is also a major hurdle for reasearchers developing drugs for Central Nervous System (CNS) disorders.
The evaluation of drug interactions in preclinical stages optimizes novel drug development
Specialized manufacturer of in vitro kits indicated to evaluate pharmacological relevant efflux and uptake receptors for TDI assays.