ReadyCell - cell based in vitro kits

Permeability Assays

Ready-to-use drug permeability kits will help you to ascertain your compound suitability for oral dosing.

Studying the permeability of compounds across a cell monolayer is a well-established in vitro technique to screen for oral absorption and evaluate transport mechanisms. Our range of pre-plated transwell products allows the rapid and accurate determination of drug transport across Caco-2 and MDCK II cell monolayers.

Your one-stop Caco-2 solution

24 or 96 transwell insert plates seeded with 21 days differentiated and polarized Caco-2 cells. The reference in vitro technique to evaluate intestinal permeability in novel orally administered compounds, according to leading regulatory agencies.

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REFERENCE	DESCRIPTION
KRECE-CCR01	CacoReady 24 Transwell
KRECE-CCR02	CacoReady 24 Well (without transwell)
KRECE-CCR04	CacoReady 24 Well (individual transwell)
KRECE-CCR50	CacoReady 96 Transwell
KRECE-CCR51	CacoReady 96 Well (without transwell)

CacoReady in the Literature

Yeste, S., et al. (2020). Preliminary in Vitro Assessment of the Potential of EST64454, a Sigma-1 Receptor Antagonist, for Pharmacokinetic Drug–Drug Interactions. Biological and Pharmaceutical Bulletin, 2020 Vol. 43:1 p. 68-76.

Aubets J, Jansat J-M, Salva M, et al. (2019). No evidence for interactions of dimethylfumarate (DMF) and its main metabolite monomethylfumarate (MMF) with human cytochrome P450 (CYP) enzymes and the P-glycoprotein (P-gp) drug transporter. Pharmacol Res Perspect. 2019;e00540.

Guardiola et al. (2018). Targeted Covalent Inhibition of Prolyl Oligopeptidase (POP): Discovery of Sulfonylfluoride Peptidomimetics. Cell Chemical Biology 25, 1031–1037.

Kenzaoui, B.H., Vilà, M., Juillerat-Jeanneret, L. (2012). Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells. Int J Nanomedicine. 2012; 7: 1275–1286. doi: 10.2147/IJN.S26865.

Pankiewicz, Piotr, et al. “Do Small Molecules Activate the TrkB Receptor in the Same Manner as BDNF? Limitations of Published TrkB Low Molecular Agonists and Screening for Novel TrkB Orthosteric Agonists.” Pharmaceuticals 14.8 (2021): 704.

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A secreting mucus intestinal barrier

24 transwell insert plates seeded with 21 days differentiated and polarized Caco-2 and HT-29 (human goblet) cells. An in vitro intestinal absorption evaluation of drug targets in a barrier physiologically closer to the intestinal epithelium.

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REFERENCE	DESCRIPTION
KRECE-CCG01	CacoGoblet 24 Transwell
KRECE-CCG02	CacoGoblet 24 (individual transwell)

CacoGoblet in the literature

Servi, B., Ranzini, F. (2017). Protective efficacy of antidiarrheal agents in a permeability model of Escherichia coli-infected CacoGoblet cells. Future Microbiology, vol. 12, no. 16. https://doi.org/10.2217/fmb-2016-0195

Fraile, B., et al. (2017). Xyloglucan, hibiscus and propolis for the prevention of urinary tract infections: results of in vitro studies. Future Microbiology, vol. 12, no. 8. https://doi.org/10.2217/fmb-2017-0015

Kenzaoui, B.H., Vilà, M., Juillerat-Jeanneret, L. (2012). Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells. Int J Nanomedicine. 2012; 7: 1275–1286. doi: 10.2147/IJN.S26865.

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An alternative cellular model based on MDCKII cells

24 and 96 transwell insert plates cultured with 12 days differentiated MDCKII parental line cells. A reference in vitro model to evaluate passive permeation and specific receptor mediated transport of new compounds.

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REFERENCE	DESCRIPTION
KRECE-CTR01	PreadyPort-CTRL (24 Transwell)
KRECE-CTR50	PreadyPort-CTRL (96 Transwell)
KRECE-MDR01	PreadyPort-MDR1 (24 Transwell)
KRECE-MDR02	PreadyPort-MDR1/CTRL 1:1 (24 Transwell)
KRECE-MDR50	PreadyPort-MDR1 (96 Transwell)
KRECE-MDR51	PreadyPort-MDR1/CTRL 1:1 (96 Transwell)

PreadyPort in the literature

Miyamoto, R., et al. (2019). The Impact of Endogenous Breast Cancer Resistance Protein on Human P-Glycoprotein-Mediated Transport Assays Using LLC-PK1 Cells Transfected With Human P-Glycoprotein. Journal of Pharmaceutical Sciences, Vol. 108, Issue 3, pp. 1085-1089. https://doi.org/10.1016/j.xphs.2018.10.012

Aubets, J., Jansat, J-M., Salva, M., et al. (2019). No evidence for interactions of dimethylfumarate (DMF) and its main metabolite monomethylfumarate (MMF) with human cytochrome P450 (CYP) enzymes and the P-glycoprotein (P-gp) drug transporter. Pharmacol Res Perspect. 2019;e00540. DOI: 10.1002/prp2.540

Mukkavilli, R., Jadhav, G., Vangala, S. (2017). Evaluation of Drug Transport in MDCKII-Wild Type, MDCKII-MDR1, MDCKII-BCRP and Caco-2 Cell Lines. Current Pharmaceutical Biotechnology, Vol. 18, No. 14, pp. 1151-1158(8). https://doi.org/10.2174/1389201019666180308091855

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ReadyCell ready-to-use cell monolayer kits benefits

No in-house cell culture maintenance nor cell licensing costs
Shipments worldwide to any location at room temperature thanks to our patented technology
High flexibility
High reproducibility within and between manufacturing batches
Cell-based assays ready-to-use
Highly predictive and useful in the in vitro to in vivo progression
Technical support during the assay and in bio-analytics

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