The use of our products manufactured with Caco-2, HT-29 and MDCKII cells will help you to investigate your compound absorption across a relevant barrier.
Cell-based assays with high flexibility and a ready-to-use system.
Highly predictive and useful in the in vitro to in vivo progression.
Worldwide shipments at room temperature thanks to our patented technology.
The CacoReady kit contains Caco-2 cells, a human colorectal cancer-derived cell line that has proven to successfully mimic the intestinal epithelial barrier.
Caco-2 cells have been extensively used over the last thirty years to study drug absorption across the human intestinal mucosa. The cells are seeded at a very specific cell number and differentiated for 21-days on transwell plates containing a permeable membrane support which results in apical and basal compartments, which artificially simulate the intestinal absorption into the bloodstream.
Caco-2 cells are considered an in vitro gold standard for assessing oral drug absorption. This model provides reliable predictive permeability data either by passive diffusion and/or active transport for hydrophilic to moderately lipophilic compounds and can easily be adapted for high-throughput screening purposes.
The Caco-2 model is also useful to assay active transport of drugs in the central nervous system (CNS) since it expresses two efflux transporters that play a relevant role in the permeation of compounds across the blood brain barrier (BBB).
The CacoGoblet model is the result of coculturing Caco-2 and HT29-MTX cells, two human colon cancer-derived cell lines that represent a barrier closer to the intestinal epithelium.
As a result of the combination of cells with an absorptive and mucus-secreting phenotype, this model possesses a mucus that differentiates it from the Caco-2 cell model. The cell barrier of this co-culture, moreover, is not as tight as that formed by Caco-2 alone and has TEER values more similar to those of the human small intestine. The tight junctions of Caco-2 cells, in contrast, more closely resemble the electrical resistance of the colon.
CacoGoblet is also considered a relevant cellular model for studies of drug absorption across the gastrointestinal barrier, either by passive diffusion and/or by active transport. The model can be easily adapted for high-throughput screening purposes. In addition to drug permeability testing CacoGoblet respond well to inflammatory mediators, providing valuable information on the efficacy of anti-inflammatory drugs.
Madin-Darby Canine Kidney Type II or MDCKII cells, included in our PreadyPort kit, are relevant to assess drug permeability by passive diffusion across a relevant barrier.
This cells were isolated from normal kidney tissue of an adult dog and they are widely used as an in vitro model of the intestinal epithelium. These cells cultured for 12-days on transwell plates containing a permeable membrane have clear apico-basolateral polarity and well defined cell junctions.
In addition, MDCKII cells are also efficient for transient and stable gene transfection, making them especially relevant to overexpress uptake and efflux drug transporters such as two members of the ATP-binding Cassette (ABC) superfamily, MDR1 and BCRP.
Studying the permeability of compounds across a cell monolayer is a well-established in vitro technique to screen for oral absorption and evaluate transport mechanisms.
Our range of pre-plated transwell products allows the rapid and accurate determination of drug transport across Caco-2, HT-29 and MDCKII cell monolayers.
Yes, we provide a concrete quality control for each batch so the customer can have a reference for the kit before and after shipment.
CacoReady and CacoGoblet are both, two cell-based kits to in vitro assay drug intestinal permeability. However, CacoReady only contains absortive epithelial cells (Caco-2 cells) while CacoGoblet is the result of coculturing Caco-2 cells and mucus-secreting cells (HT29-MTX cells), bringing it closer to the most physiological conditions.
Because they consist of different cell phenotypes, the barrier properties are also different being very tight for CacoReady with TEER values ≥ 1000 ohms x cm2 and looser for CacoGoblet (≥ 70 ohms x cm2), being the drug permeability assays for both models very consistent.
No, ReadyCell’s Shipping Medium consists of a semi-solid culture system specifically designed to preserve cells at room temperature (15-25ºC). This medium maintains a suitable physicochemical environment, keeping adequate moisture conditions for cellular homeostasis and forming a protective cushion that protects cell integrity and functionality during long-distance shipments up to seven days.
The integrated Transwell format is recommended for permeability assays by reference, since it enableds an apical-basal polarization. The non-Transwell format is used for other types of tests, including toxicity or cell culture for measurement of protein expression. The individual Transwell is recommended for permeability tests which require the individual mobility of each Transwell for different times or measurements.
According to the European and the American Regulatory Agencies (EMA and FDA), permeability assays employing cultured Caco-2 are the reference model to estimate intestinal drug absorption in humans.
The reference compounds used in these tests are categorized as high permeability, low permeability and P-gp substrate. In the case of CacoReady, the main reference high permeability compound is metoprolol (Papp A-B >10 cm/s) and for the low, atenolol (Papp A-B <0.5 cm/s). Digoxin is used as P-gp substrate with a Papp A-B <1 cm/s.
Inflammatory reactions of the intestinal tract are the result of a combination of hereditary, genetic and/or environmental factors that have been clustered in the inflammatory bowel disease (IBD). ReadyCell has available CacoReady and CacoGoblet to assay compounds inflammatory properties.
A new drug against malignant cells was found using methods such as High Throughput Screening (HTS) and Caco-2 permeability assay with CacoReady plates.
The presence of the superfamily members of the ATP binding cassette (ABC) transporters is well established as the main cause of multidrug resistance, since they efflux therapeutic compounds from cells and reduce the intracellular drug levels.
