CELL PERMEABILITY ASSAY MODELS
Ready-to-use cells for preclinical stages
Caco-2 permeability assay
Caco-2 cells are considered an in vitro gold standard for assessing oral drug absorption. This model provides reliable predictive permeability data either by passive diffusion and/or active transport for hydrophilic to moderately lipophilic compounds and can easily be adapted for high-throughput screening purposes.
The Caco-2 model is also useful to assay active transport of drugs in the central nervous system (CNS) since it expresses two efflux transporters that play a relevant role in the permeation of compounds across the blood brain barrier (BBB).
Caco-2 and HT-29 permeabilty assay
CacoGoblet is also considered a relevant cellular model for studies of drug absorption across the gastrointestinal barrier, either by passive diffusion and/or by active transport. The model can be easily adapted for high-throughput screening purposes. In addition to drug permeability testing CacoGoblet respond well to inflammatory mediators, providing valuable information on the efficacy of anti-inflammatory drugs.
MDCKII permeability assay
In addition, MDCKII cells are also efficient for transient and stable gene transfection, making them especially relevant to overexpress uptake and efflux drug transporters such as two members of the ATP-binding Cassette (ABC) superfamily, MDR1 and BCRP.
ReadyCell permeability kits
We help you to determine your compound suitability for oral dosing
Studying the permeability of compounds across a cell monolayer is a well-established in vitro technique to screen for oral absorption and evaluate drug transporters.
Our range of pre-plated transwell products allows the rapid and accurate determination of drug transport across Caco-2, HT-29 and MDCKII cell monolayers.
Frequently Asked Questions
Is the quality control data specific for each kit?
What is the difference between CacoReady and CacoGoblet?
Because they consist of different cell phenotypes, the barrier properties are also different being very tight for CacoReady with TEER values ≥ 1000 ohms x cm2 and looser for CacoGoblet (≥ 70 ohms x cm2), being the drug permeability assays for both models very consistent.