Permeability assays are important for understanding how molecules cross biological barriers, assessing both their ability to cross and the mechanisms involved. While drugs are often absorbed by passive diffusion, some molecules can also be substrates for membrane proteins or transporters.
The permeability assay protocol for Caco-2 cells serves as a cornerstone in understanding and predicting the intestinal absorption of pharmaceutical agents.
In a recent publication in the Journal of Medicinal Chemistry, a collaborative research led by the scientist Dr. H. Day, in conjunction with a consortium of researchers, explored the development of allosteric inhibitors targeting SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) for cancer therapy.
The article highlights the discovery of a series of azetidine derivatives, known as BGAz compounds, that exhibit potent bactericidal activity against both drug-sensitive Mycobacterium tuberculosis and multidrug-resistant TB strains.
In a recent study published in the Future Pharmacology Journal, researchers from prestigious French companies examined the impact of four polyols on the permeability of seven active pharmaceutical ingredients (APIs).
Evaluating in vitro permeability with CacoReady plates was central to the study design. By quantifying the permeability of the synthesized compounds within Caco-2 cells, researchers could identify and select which compounds were best in terms of cellular adsorption.
