Permeability assays are important for understanding how molecules cross biological barriers, assessing both their ability to cross and the mechanisms involved. While drugs are often absorbed by passive diffusion, some molecules can also be substrates for membrane proteins or transporters.
The permeability assay protocol for Caco-2 cells serves as a cornerstone in understanding and predicting the intestinal absorption of pharmaceutical agents.
In preclinical research, the choice of the appropriate well system format – whether it’s a transwell, non-transwell, or individual transwell – can significantly impact the outcome of experiments. Selecting the correct plate is crucial to working with permeability, toxicity, drug screening, or other assays.
This innovative biotechnology is available to transport living cells at room temperature.
Last month, the ICH launched a draft on Drug Interaction Studies regulatory. The guideline includes the regulations of the European, American and Japanese medical agencies and covers both in vitro and clinical studies.
Among other things, the agency explains that DDIs are a critical factor in a drug’s overall benefit-risk profile.
